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maxi script kit  (Thermo Fisher)


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  • 90

    Structured Review

    Thermo Fisher maxi script kit
    Maxi Script Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxi+script+kit/pm38133285-63-17-20
    Average 90 stars, based on 1 article reviews
    maxi script kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: Nuclease Activity of Legionella pneumophila Cas2 Promotes Intracellular Infection of Amoebal Host Cells
    Article Snippet: Control reactions utilizing RNase A (Life Technologies) confirmed that the substrates and assay conditions were working as expected (data not shown). .. In order to examine the effect of Cas2 on long ssRNA, pTRI-actin, encoding a 304-nucleotide (nt) portion of the mouse β-actin gene (MAXI script kit; Ambion, Austin, TX), and bacteriophage λ DNA, encoding a 523-nt portion of the phage genome (Jena Biosciences, Jena, Germany), were templates in in vitro transcription reactions using an Atto-680 RNA labeling kit (Jena Biosciences). .. The fluorescently labeled molecules were detected by the LI-COR system.

    Article Title: Identification of a Novel Hepacivirus in Southeast Asian Shrew ( Crocidura fuliginosa ) from Yunnan Province, China.
    Article Snippet: Using a HiScript II One Step qRT-PCR SYBR Green kit (Vazyme Biotech, Nanjing, China), quantitative RT-PCR was performed according to the manufacturer’s instructions. .. A positive control from the partial genome RNA transcript of the DNA template was applied using a MAXI script Kit (Applied Biosystems, Waltham, MA, USA) in vitro. ..

    Article Title: Identification of a Novel Hepacivirus in Southeast Asian Shrew ( Crocidura fuliginosa ) from Yunnan Province, China
    Article Snippet: Using a HiScript II One Step qRT-PCR SYBR Green kit (Vazyme Biotech, Nanjing, China), quantitative RT-PCR was performed according to the manufacturer’s instructions. .. A positive control from the partial genome RNA transcript of the DNA template was applied using a MAXI script Kit (Applied Biosystems, Waltham, MA, USA) in vitro. ..

    Article Title: Molecular characterization, tissue distribution, and ultrastructural localization of adipokinetic hormones in the CNS of the firebug Pyrrhocoris apterus (Heteroptera, Insecta).
    Article Snippet: http://dx.doi.org/10.1016/j.ygcen.2014.10.014 0016-6480/ 2014 Elsevier Inc. All rights reserved.. ⇑ Corresponding author at: Institute of Entomology, Biology Centre, Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic.. E-mail address: kodrik@entu.cas.cz (D. Kodrík).

    Article Title: The Proinflammatory Cytokine, Interleukin-6, Up-regulates Calcium-sensing Receptor Gene Transcription via Stat1/3 and Sp1/3
    Article Snippet: For the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) riboprobe that protects a 316-nucleotide transcript, the pTRI-GAPDH Rat vector (AM7432: Ambion Inc., Austin, TX) was used. .. After linearization of the vectors, the antisense probeswere in vitro transcribedwithT7 polymerase incorporating [ -32P]UTP using a MAXI script kit, and the gel-purified riboprobes were used with a ribonuclease protection analysis II kit (Ambion Inc.). ..

    Labeling:

    Article Title: Nuclease Activity of Legionella pneumophila Cas2 Promotes Intracellular Infection of Amoebal Host Cells
    Article Snippet: Control reactions utilizing RNase A (Life Technologies) confirmed that the substrates and assay conditions were working as expected (data not shown). .. In order to examine the effect of Cas2 on long ssRNA, pTRI-actin, encoding a 304-nucleotide (nt) portion of the mouse β-actin gene (MAXI script kit; Ambion, Austin, TX), and bacteriophage λ DNA, encoding a 523-nt portion of the phage genome (Jena Biosciences, Jena, Germany), were templates in in vitro transcription reactions using an Atto-680 RNA labeling kit (Jena Biosciences). .. The fluorescently labeled molecules were detected by the LI-COR system.

    Article Title: Molecular characterization, tissue distribution, and ultrastructural localization of adipokinetic hormones in the CNS of the firebug Pyrrhocoris apterus (Heteroptera, Insecta).
    Article Snippet: http://dx.doi.org/10.1016/j.ygcen.2014.10.014 0016-6480/ 2014 Elsevier Inc. All rights reserved.. ⇑ Corresponding author at: Institute of Entomology, Biology Centre, Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic.. E-mail address: kodrik@entu.cas.cz (D. Kodrík).

    Article Title: Molecular analyses of the gill symbiosis of the bathymodiolin mussel Gigantidas platifrons
    Article Snippet: .. Digoxigenin labeled anti-sense probes or sense control probe were transcribed using the DIG labeling mix (Roche) and Maxi Script kit with either T7 or Sp6 enzyme (Ambion) according to the manufacturer's protocol, and then cleaned up using SigmaSpin post reactive cleanup column (Sigma-Aldrich). ..

    Positive Control:

    Article Title: Identification of a Novel Hepacivirus in Southeast Asian Shrew ( Crocidura fuliginosa ) from Yunnan Province, China.
    Article Snippet: Using a HiScript II One Step qRT-PCR SYBR Green kit (Vazyme Biotech, Nanjing, China), quantitative RT-PCR was performed according to the manufacturer’s instructions. .. A positive control from the partial genome RNA transcript of the DNA template was applied using a MAXI script Kit (Applied Biosystems, Waltham, MA, USA) in vitro. ..

    Article Title: Identification of a Novel Hepacivirus in Southeast Asian Shrew ( Crocidura fuliginosa ) from Yunnan Province, China
    Article Snippet: Using a HiScript II One Step qRT-PCR SYBR Green kit (Vazyme Biotech, Nanjing, China), quantitative RT-PCR was performed according to the manufacturer’s instructions. .. A positive control from the partial genome RNA transcript of the DNA template was applied using a MAXI script Kit (Applied Biosystems, Waltham, MA, USA) in vitro. ..

    Control:

    Article Title: Molecular analyses of the gill symbiosis of the bathymodiolin mussel Gigantidas platifrons
    Article Snippet: .. Digoxigenin labeled anti-sense probes or sense control probe were transcribed using the DIG labeling mix (Roche) and Maxi Script kit with either T7 or Sp6 enzyme (Ambion) according to the manufacturer's protocol, and then cleaned up using SigmaSpin post reactive cleanup column (Sigma-Aldrich). ..

    Activity Assay:

    Article Title: Leptin Induces Increased ?2(I) Collagen Gene Expression in Cultured Rat Hepatic Stellate Cells
    Article Snippet: Two micrograms of total RNA from untreated HSCs was reverse-transcribed to cDNA using RETROscript First Strand Synthesis Kit for RT-PCR (Ambion, Austin, TX) as recommended by the manufacturer. .. Subsequently, a 533-nucleotide antisense riboprobe was transcribed with T7RNA polymerase (MAXI script kit, Ambion) by using [α 32 P] uridine triphosphate (specific activity, 800 Ci/mmol) [Amersham Pharmacia Biotech, Piscataway, NJ]. .. Subsequently, a 533-nucleotide antisense riboprobe was transcribed with T7RNA polymerase (MAXI script kit, Ambion) by using [α 32 P] uridine triphosphate (specific activity, 800 Ci/mmol) [Amersham Pharmacia Biotech, Piscataway, NJ].



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